Research Article

Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH

Figure 5

Time-resolved spectra for the reaction of hDDC with L-Dopa. Rapid scanning stopped-flow spectra obtained upon reaction of hDDC (20 μM) with L-Dopa (2 mM) in Tris-Bis-propane, pH 7.4, at 25°C. Spectra were taken between 1 and 100 ms at 1 ms intervals and between 101 and 200 ms at 10 ms intervals.
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