Interaction of Human Dopa Decarboxylase with L-Dopa: Spectroscopic and Kinetic Studies as a Function of pH

Figure 6

pH dependence of the amplitude of the change of the 390 nm absorbance band in the reaction of hDDC with L-Dopa. Amplitude of the 390 nm absorbance band monitored by rapid scanning stopped-flow spectra upon reaction of hDDC (20 μM) and L-Dopa (2 mM) in Tris-Bis-propane, at 25°C at the indicated pH. The points shown are the experimental values, while the curve is from fit to the data using (3).