In Silico Prediction and In Vitro Characterization of Multifunctional Human RNase3
Figure 8
Binding activity of hRNase3 and to wild-type and mutant CHO cell lines. Cellular binding activity of hRNase3 (a) and FITC- (b) to CHO-K1, CHO-pgsD677, and CHO-pgsA745 cells were assayed using cELISA. The amount of 1000 nM hRNase3 and 5 μM FITC- bound to wild-type CHO-K1cell was set as 100% binding, respectively. The data represented the mean value of triple independent experiments and the error bar was shown as standard deviation (SD).
