Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem

Table 1

Morphological and biochemical properties of Bacillus sp. MA20 and Bacillus sp. MA21.

Characteristics	Bacillus sp. MA20	Bacillus sp. MA21
Morphological
Shape	Rods	Rods
Gram stain	G+ve	G+ve
Motility	Motile	Motile
spore formation	+ve	+ve
Growth
Growth temperature	15°C–50°C	15°C–60°C
Growth pH	5–8	5–8
Biochemical tests
Oxidase	+ve	+ve
Catalase	+ve	+ve
Voges-Proskauer	+ve	+ve
Indol production	−ve	−ve
Nitrate reduced to nitrite	+ve	+ve
Hydrolysis of
Casein	+ve	+ve
Gelatin	+ve	+ve
Wool	+ve	+ve
Starch	+ve	+ve
Acid from
Glucose	+ve	+ve
Arabinose	+ve	+ve
Xylose	+ve	+ve
Mannitol	+ve	+ve
Gas from glucose	−ve	−ve
Utilization of
Citrate	+ve	+ve
Propionate	−ve	−ve