Research Article
Production and Characterization of Keratinolytic Protease from New Wool-Degrading Bacillus Species Isolated from Egyptian Ecosystem
Table 2
Effect of protease inhibitors, metal ions, and solvents on proteolytic activity of B. amyloliquefaciens MA20.
| Substance | Final concentration | Relative activity (%) | Final concentration | Relative activity (%) |
| Control | 0 | 100 | 0 | 100 | β-mercaptoethanol | 5 mM | 18 | 1 mM | 98 | PMSF | 5 mM | 0 | 1 mM | 0 | EDTA | 5 mM | 35 | 1 mM | 97 | SDS | 0.5% | 54 | 0.1% | 122 | ZnCl2 | 10 mM | 153 | 5 mM | 156 | MgCl2 | 10 mM | 119 | 5 mM | 95 | CuSO4 | 10 mM | 266 | 5 mM | 204 | Urea | 10 mM | 114 | 5 mM | 139 | HgCl2 | 10 mM | 100.4 | 5 mM | 107 | CaCl2 | 10 mM | 104 | 5 mM | 107 | BaCl2 | 10 mM | 115 | 5 mM | 116 | Guanidin HCl | 10 mM | 103 | 5 mM | 132 | MnCl2 | 10 mM | 71 | 5 mM | 162 | Methanol | 1% | 90 | 0.5% | 100 | Ethanol | 1% | 103 | 0.5% | 100 | DMSO | 1% | 90 | 0.5% | 103 | Isopropanol | 1% | 98 | 0.5% | 108 | Tween 20 | 1% | 86 | 0.5% | 94 | Triton X100 | 1% | 61 | 0.5% | 65 |
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