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BioMed Research International
Volume 2013, Article ID 176946, 12 pages
http://dx.doi.org/10.1155/2013/176946
Research Article

The Influence of Chain Microstructure of Biodegradable Copolyesters Obtained with Low-Toxic Zirconium Initiator to In Vitro Biocompatibility

1Department of Biopharmacy, School of Pharmacy, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland
2Centre of Polymer and Carbon Materials, Polish Academy of Sciences, M. Curie Sklodowskiej 34, 41-819 Zabrze, Poland
3Department of Biomaterials, AGH University of Science and Technology, Mickiewicza 30, 30-059 Krakow, Poland
4Department of Molecular Biology, Medical University of Silesia, Narcyzów 1, 41-200 Sosnowiec, Poland
5Department of Pediatric Surgery, Medical University of Silesia, Medyków 16, 40-752 Katowice, Poland

Received 26 April 2013; Accepted 12 July 2013

Academic Editor: Jean-Pierre Molès

Copyright © 2013 Arkadiusz Orchel et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Abstract

Because of the wide use of biodegradable materials in tissue engineering, it is necessary to obtain biocompatible polymers with different mechanical and physical properties as well as degradation ratio. Novel co- and terpolymers of various composition and chain microstructure have been developed and applied for cell culture. The aim of this study was to evaluate the adhesion and proliferation of human chondrocytes to four biodegradable copolymers: lactide-coglycolide, lactide-co- -caprolactone, lactide-co-trimethylene carbonate, glycolide-co- -caprolactone, and one terpolymer glycolide-colactide-co- -caprolactone synthesized with the use of zirconium acetylacetonate as a nontoxic initiator. Chain microstructure of the copolymers was analyzed by means of 1H and 13C NMR spectroscopy and surface properties by AFM technique. Cell adhesion and proliferation were determined by CyQUANT Cell Proliferation Assay Kit. After 4 h the chondrocyte adhesion on the surface of studied materials was comparable to standard TCPS. Cell proliferation occurred on all the substrates; however, among the studied polymers poly(L-lactide-coglycolide) 85 : 15 that characterized the most blocky structure best supported cell growth. Chondrocytes retained the cell membrane integrity evaluated by the LDH release assay. As can be summarized from the results of the study, all the studied polymers are well tolerated by the cells that make them appropriate for human chondrocytes growth.