Figure 7: Overexpression of NUMB-GFP in HAT-7 cells: (a) Western blot analysis to detect overexpression of NUMB-GFP in HAT-7 cells. Total proteins were isolated from 2 clones, namely, clone 1 and 2. (b) Immunohistochemical analysis detected that Notch1 protein expression is dramatically reduced in the NUMB overexpressing cell line (stable cell line clone 2 was used). The green fluorescence represents activated Notch1 protein.