Analysis of Pregnancy-Associated Plasma Protein A Production in Human Adult Cardiac Progenitor Cells
Figure 3
Immunofluorescence and confocal analysis of PAPP-A in CSps and CDCs. Representative confocal images for the detection of PAPP-A in CSps (a–d) and CDCs (e-f) by immunofluorescence: PAPP-A/proMBP complex and free PAPP-A are stained by A0230; homodimeric PAPP-A is recognized by 4PD4 antibody and pro-MBP by 5H9. Note that (a–d) display different focal planes (core or surface) of the same CSp, showing how the spatial distribution of dPAPP-A and proMBP, respectively, varies inside the 3D structure of the CSp. A higher magnification panel of the corresponding evidenced area is shown as insert in (e).
