BioMed Research International / 2013 / Article / Tab 2 / Review Article
The Impact of Hepatic Steatosis on Hepatic Ischemia-Reperfusion Injury in Experimental Studies: A Systematic Review Table 2 Histological findings in experimental models of hepatic warm ischemia-reperfusion injury and hepatic steatosis.
Author Animal Steatosis model % steatosis Type of steatosis Duration of ischemia (mins, type) Duration of reperfusion (mins) Outcome measures Results in steatotic livers (lean livers) Yamada et al. [38 ] Rat D-C 30–60 MiS 30 (partial) 240 HIS 5 (5)1 Yamada et al. [39 ] Rat D-C 30–60 MiS 30 (partial) 24 hours HIS 6 (6)1 Ellett et al. [29 ] Mouse HFD 30–60 MaS 35 (total) 24 hours HIS 1.4 ± 0.1 (0.4 ± 0.1)2 Yamagami et al. [30 ] Rat3 CDD 40–60 MaS 45 (total) 40 or 180 Histo Severe congestion and necrosis He et al. [31 ] Mouse HFD 50–60 MaS 45 (total) 24 hours HIS 1.8 ± 0.2 (0.9 ± 0.1)2 Marsman et al. [40 ] Rat CMDD >60 MaS 40 (total) 24 hours Histo (% necrosis) 37 ± 10 (5 ± 1%)2 Andraus et al. [41 ] Rat PFD >60 MaS 60 (partial) 240 Histo
Intraparenchymal haemorrhageSelzner et al. [33 ] Mouse CDD >60 Mixed 45 or 60 (partial) 24 hours Histo (% necrosis) 65 ± 24% (25 ± 12%)2 Caraceni et al. [34 ] Rat CDD >60 MaS 60 30 to 24 hours Histo
Sinusoidal congestion and necrosisHakamada et al. [35 ] Rat CDD >60 MaS 30 or 60 (total) 360 Histo
Sinusoidal congestion and necrosisHui et al. [36 ] Rat CDD >70 MaS 30, 45, or 60 60 Histo Severe necrosis and haemorrhage (no morphological change) Takahashi et al. [37 ] Canine HFD 7–99 MaS 60 24 hours Histo
Sinusoidal congestion and necrosis
CDD: choline-deficient diet; CMDD: choline-methionine-deficient diet; D-C: dextrose with cholesterol; HFD: high-fat diet; HIS: histological injury score; Histo: histology; MaS: macrovesicular steatosis; MiS: microvesicular steatosis; Mixed: presence of both macrovesicular and microvesicular steatosis; PFD: protein-free diet.
No significant difference between steatotic and lean livers;
P < 0.05 versus lean livers;
no lean group in study.