Review Article

Quantification of Human and Animal Viruses to Differentiate the Origin of the Fecal Contamination Present in Environmental Samples

Table 3

JCPyV (or HPyV) quantification studies in environmental water matrices.

Authors [Reference]qPCR detection method [Reference]Matrices analyzedMain results

Albinana-Gimenez et al., 2006 [36]Pal et al., 2006 [27]Sewage and river 100% positive samples in sewage (5/5) and river (9/9). Mean values and  GC/L, respectively
Bofill-Mas et al., 2006 [37]Pal et al., 2006 [27]Sewage, effluent, and sludge99% positive samples. T99 of 127.3 days
Albinana-Gimenez et al., 2009 [62]Pal et al., 2006 [27]River48% positive samples in river water
Albinana-Gimenez et al., 2009 [39] Pal et al., 2006 [27] River and drinking-water treatment plant (DWTP)48% positive samples (different steps of the DWTP ) with mean values to  GC/L
McQuaig et al., 2009 [63]McQuaig et al., 2009 [63]Sewage, fresh to marine water, animal wasteMean values in sewage  GC/L
Hamza et al., 2009 [42]Biel et al., 2000 [64]River Detected (as JC and BK) in 97.5% of the samples
Harwood et al., 2009 [65] McQuaig et al., 2009 [63] River, animal feces, and seawater No detection of HPyV in animal feces
No correlation with Enterococcus
100% host specificity
Ahmed et al., 2009 [66]McQuaig et al., 2009 [63]Sewage
Abdelzaher et al., 2010
[67]
McQuaig et al., 2009 [63]SeawaterThe FIB levels exceeded regulatory guidelines during one event, and this was accompanied by detection of HPyVs and pathogens
Ahmed et al., 2010 [68]McQuaig et al., 2009 [63]Sewage and seawater JC and BK are highly host-specific viruses and high titers are found in sewage
Bofill-Mas et al., 2010 [44]Pal et al., 2006 [27]River and sewageSewage ranges from to  GC/L (7/7)
River ranges from to  GC/L (7/7)
Fumian et al., 2010 [69]Pal et al., 2006 [27]Sewage and effluentJCPyV detected in 96% and 43% of raw and treated sewage, respectively
Haramoto et al., 2010 [45]Pal et al., 2006 [27]River JCPyV prevalence 11.1%, BKPyV not detected
Jurzik et al., 2010 [46]Biel et al., 2000 [64], and modified by Hamza et al., 2009 [42]River68.8% were positive for HPyV
Gibson et al., 2011 [70]McQuaig et al., 2009 [63]River and drinking waterHPyV were detected in one groundwater, three-surface water, and one drinking-water sample. No correlation with FIB
Hamza et al., 2011 [54]Biel et al., 2000 [64]River and sewageRiver  GC/L, sewage  GC/L
Hellein et al., 2011 [71]McQuaig et al., 2009 [63]Seawater, sewage, and animal fecesPresence of HPyV in all sewage samples and in one freshwater sample
Kokkinos et al., 2011 [55]McQuaig et al., 2009 [63]Sewage68.8% positive values (33/48) for JC and BK
Wong and Xagoraraki, 2011 [72]McQuaig et al., 2009 [63]Manure sewage and sludge HPyV concentrations were slightly lower than Escherichia coli and Enterococcus ( )
Chase et al., 2012 [73]McQuaig et al., 2009 [63]Recreational waters HPyV detection near septic systems
Fongaro et al., 2012 [60]McQuaig et al., 2009 [63]Lagoon21% positive samples
Gordon et al., 2013 [74]McQuaig et al., 2009 [63]Estuarine to marine waters and sewage spillsHPyV demonstrated the ability to detect domestic sewage contamination in water
Rodriguez-Manzano
et al., 2012 [13]
Hernroth et al., 2002 [26]Raw sewage, secondary and tertiary effluentJCPyV in raw sewage (6/6) with an average concentration of  GC/L. Not detected in the tertiary effluent.
McQuaig et al., 2012 [75]McQuaig et al., 2009 [63]SeawaterMean values to  GC/L
Staley et al., 2012 [76]Staley et al., 2012 [76]Sewage, river100% and 64% positive samples of sewage and river samples, respectively