Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation
Effect of 4-1BB siRNA transduction on the expression of 4-1BB in the splenic lymphocytes. The recombination vector of lentivirus containing shRNA targeting the 4-1BB gene was constructed. Lymphocytes from BN rats were infected by recombinant LVs. ddH2O was used as the control. (a) Real-time PCR analysis of the mRNA level of 4-1BB gene in the splenic lymphocytes 72 h after transduction. (b and c) The protein level of 4-1BB gene in the splenic lymphocytes was showed by Western blot analysis 72 h after transduction. β-actin was used as an internal standard. LV-NC: negative-control shRNA group; LV-sh4-1BB1-3: pcDNA-CMV-sh4-1BB1-3-Lentivector group. Data were expressed as means SD. compared with the LV-NC group.