Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation
Effect of lentivirus-mediated delivery of shRNA targeting 4-1BB gene on 4-1BB expression of the splenic lymphocytes isolated from the recipient rats with liver transplantation. The recombination vector of lentivirus contains shRNA targeting the 4-1BB gene was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The splenic lymphocytes from the recipient rats were gathered 7 days after transduction. Sham was used as the control. (a) Real-time PCR analysis of the mRNA level of 4-1BB gene in the splenic lymphocytes 7 days after transduction. (b and c) The protein level of 4-1BB gene in the splenic lymphocytes was showed by Western blot analysis 7 days after transduction. β-actin was used as an internal standard. M: the liver transplantation group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB1-Lentivector group. Data were expressed as means SD. compared with the sham (control) group; compared with the LV-NC group.