Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation
Effect of lentivirus-mediated delivery of shRNA targeting 4-1BB gene on the survival time in BN recipients rats after liver transplantation. The recombination vector of lentivirus contains shRNA targeting the 4-1BB gene (LV-sh4-1BB1) was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The survival time of recipients rats was recorded and the percentage of survival was calculated. M: the liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB1-Lentivector group. Data were expressed as means SD, . compared with the LV-NC group.