Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation

<table>Effect of lentivirus-mediated delivery of shRNA targeting 4-1BB gene on the survival time in BN recipients rats after liver transplantation. The recombination vector of lentivirus contains shRNA targeting the 4-1BB gene (LV-sh4-1BB1) was constructed. The liver transplantation was performed using the two-cuff technique. BN recipient rats were infected by the recombinant LVs. The survival time of recipients rats was recorded and the percentage of survival was calculated. M: the liver transplantation model group; LV-NC: negative-control shRNA group; LV-sh4-1BB1: pcDNA-CMV-sh4-1BB1-Lentivector group. Data were expressed as means <svg height="9.5625" id="M21" style="vertical-align:-0.3003pt" version="1.1" viewbox="0 0 10.1 9.5625" width="10.1" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
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</svg> compared with the LV-NC group.</table>

BioMed Research International

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Figure 5

Figure 5: Gene Silencing of 4-1BB by RNA Interference Inhibits Acute Rejection in Rats with Liver Transplantation 