Metabolomic Analysis of Differential Changes in Metabolites during ATP Oscillations in Chondrogenesis
Figure 1
ATP oscillations are linked with both glycolysis and mitochondrial respiration. Either a glycolysis inhibitor iodoacetate or an inhibitor of mitochondrial respiration cyanide eliminates insulin-induced PACTIN-PxRe oscillations in ATDC5 cells. Bioluminescence monitoring of PACTIN-PxRe activity in ATDC5 cells was performed after chondrogenic induction with the chondrogenic medium ( h). About 24 hours after PACTIN-PxRe oscillations were initiated, ATDC5 cells were treated with iodoacetate or cyanide.
