BioMed Research International / 2013 / Article / Fig 2

Research Article

PPAR Activation Induces M1 Macrophage Polarization via cPLA2-COX-2 Inhibition, Activating ROS Production against Leishmania mexicana

Figure 2

Cytokine determination in L. mexicana-infected macrophages. Levels of gene expression for each sample were normalized with β-actin RNA as internal control. Modulation was expressed relative to the untreated control using the method. The -axis intercepts the -axis at “1” to show the increase and the decrease of each cytokine compared to nontreated infected macrophages. Relative expression level for each cytokine was calculated according to = (CT test − CT  β-actin) treated − (CT test − CT  β-actin) untreated formula [17]. Graph bars are mean ± SEM of three independent experiments, and statistical analysis was done comparing, for each time, treated versus nontreated macrophages; (*) , (**) , and (***) .

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