Figure 4: Phagocytic activity of J774A.1 macrophages was determined for zymosan and L. mexicana promastigotes uptake; treated or nontreated macrophages were incubated with zymosan-FITC for 2 h or infected with CFSE-promastigotes for 1-2 h. (a) Phagocytizing macrophage percentage. (b) Zymosan particles or parasites/cell (MFI). Graph bars are mean ± SEM of three independent experiments and statistical analysis was done comparing, for each time, treated versus nontreated macrophages; (*) , (**) , (***) .