Figure 4: Expression of trophoblast (CDX2), cell stypo determining (NOTCH1), and core “stemmness-” associated (OCT4, NANOG, and SOX2) stem cell transcription factors in JEG3 cells. JEG3 cells express CDX2 and NOTCH1 ((a) and (i)). OCT4, NANOG and SOX2 signaling are not visible (with human serum: (e), (m), and (q); without human serum (f), (n), and (r)). Immunofluorescent staining seems less specific with or without blockage with human serum (all positive signals: (c), (d), (g), (h), (k), (l), (o), (p), (s), and (t)).