Research Article | Open Access
In Situ Gene Mapping of Two Genes Supports Independent Evolution of Sex Chromosomes in Cold-Adapted Antarctic Fish
Two genes, that is, 5S ribosomal sequences and antifreeze glycoprotein (AFGP) genes, were mapped onto chromosomes of eight Antarctic notothenioid fish possessing a X1X1X2X2/X1X2Y sex chromosome system, namely, Chionodraco hamatus and Pagetopsis macropterus (family Channichthyidae), Trematomus hansoni, T. newnesi, T. nicolai, T. lepidorhinus, and Pagothenia borchgrevinki (family Nototheniidae), and Artedidraco skottsbergi (family Artedidraconidae). Through fluorescence in situ hybridization (FISH), we uncovered distinct differences in the gene content of the Y chromosomes in the eight species, with C. hamatus and P. macropterus standing out among others in bearing 5S rDNA and AFGP sequences on their Y chromosomes, respectively. Both genes were absent from the Y chromosomes of any analyzed species. The distinct patterns of Y and non-Y chromosome association of the 5S rDNA and AFGP genes in species representing different Antarctic fish families support an independent origin of the sex heterochromosomes in notothenioids with interesting implications for the evolutionary/adaptational history of these fishes living in a cold-stable environment.
In fishes the processes that influence the definition of sex (sex determination) may be subject to intrinsic genetic control, environmental control, or a combination of both [1, 2]. When only genetic factors influence the direction of sex determination, a genetic system of sex determination (GSD) is active. In this case, the chromosomes bearing the sex determining gene/s can be defined as “sex chromosomes.” These chromosomes derived from proto-sex chromosomes (autosomes that supposedly contain a cluster of closely linked sex genes) mainly through suppression of recombination between male and female regions leading to chromosomal sex inheritance pattern (e.g., [2–6]). During their evolutionary history, the sex chromosomes might become morphologically distinct (heteromorphic) as the result of the extension of the region where recombination is suppressed, along with chromosomal changes often accompanied by heterochromatin increase. The presence of heteromorphic sex-related chromosomes, recognizable in a given species, is strongly indicative of genetic control of sex determination acting in that species over other theoretical sex control systems [1, 7, 8].
In fishes sex chromosomes are not often recognizable based on gross morphology, and only a minority of species show visible sex-specific heteromorphic chromosomes [1, 10–13]. In contrast, a surprisingly high frequency of morphologically differentiated sex chromosomes occur in Notothenioidei, a perciform fish group endemic to Antarctic waters [14, 15].
Of the approximately 130 notothenioid species in eight families known to date, 123 species and five families (Harpagiferidae, Nototheniidae, Artedidraconidae, Bathydraconidae, and Channichthyidae) form an Antarctic clade  and dominate the ichthyofauna of High Antarctic regions in terms of species number and biomass . The evolution of the Antarctic notothenioid fishes in geographical isolation and in a chronically cold marine environment resulted in stenothermality of the extant species, and in the acquisition of a suite of adaptive modifications (e.g., [17–19]). Indeed, the species of the Antarctic notothenioid clade are able to live and thrive in the freezing and icy Antarctic waters thanks to remarkable cold adaptations, one of the most striking being the capability to produce antifreeze glycoproteins (AFGPs) [20–22].
The main aim of the present study was to provide a first characterization of the sex chromosomes of Antarctic notothenioid fishes in terms of gene content. We report here on the mapping of two genes, that is, 5S ribosomal sequences and antifreeze glycoprotein (AFGP) genes, onto the chromosomes of eight Antarctic notothenioid species, namely, Chionodraco hamatus and Pagetopsis macropterus (family Channichthyidae), Trematomus hansoni, T. newnesi, T. nicolai, T. lepidorhinus, and Pagothenia borchgrevinki (family Nototheniidae), and Artedidraco skottsbergi (family Artedidraconidae), showing multiple sex chromosome systems of the X1X1X2X2/X1X2Y type. We uncovered differences in the gene content of the Y chromosomes in the eight probed species, referable to three patterns of Y and non-Y chromosome association of the 5S rDNA and AFGP genes. Remarkably, 5S rDNA sequences were found into the Y chromosome in the species C. hamatus and AFGP gene clusters were located on the Y chromosome of P. macropterus. Our results are the first information on the gene content of sex chromosomes in these cold-adapted fishes.
2. Materials and Methods
2.1. Animal Sampling and Chromosome Preparation
Specimens were collected in the Ross Sea and Adélie Land coastal areas during Italian, American, and French Antarctic expeditions, from 2000 to 2010. Sites and size of sampling are summarized in Table 1.
Fishes were kept in aquaria with running, aerated seawater, and treated in vivo with colchicine. Mitotic somatic cells were obtained from head kidney and spleen, following standard protocols for direct chromosome preparations in Antarctic fishes, fixed in 3/1 methanol/acetic acid (v/v), and stored at −20°C for later analyses.
The experiments followed ethical protocols and anesthesia was administered prior to sacrificing the animals.
Voucher specimens of every studied species are conserved at the National Natural History Museum (MNHN, Paris) and at the Antarctic National Museum (University of Genoa).
2.2. FISH Probes
The 5S ribosomal probe corresponds to a partial sequence (87 bp) of the highly conserved 5S rRNA coding region, obtained from the genomic DNA of Chionodraco hamatus as described by Ghigliotti et al. .
A DNA fragment (1218 bp), obtained from the notothenioid Dissostichus mawsoni shotgun library of the BAC plasmid DmBAC64 (, AFGP gene H2A7 in GenBank accession number HQ440760), and encoding for the repetitive (Thr-Ala/Pro-Ala)n AFGP polyprotein sequence, was used as the AntiFreeze GlycoProtein (AFGP) probe.
The 5S rDNA and the AFGP probes were nick translation labeled with biotin-16-dUTP (Roche) according to standard procedures, ethanol purified, and dissolved in the hybridization buffer (50% formamide/2X SSC, 40 mM, KH2PO4, 10% dextran sulphate) to a final concentration of 15 ng/μL and 20 ng/μL respectively.
2.3. Fluorescence In Situ Hybridization
The chromosomal preparations, aged for two days at room temperature, were denatured by heating at 70°C for 1 min in 70% (v/v) formamide/2X SSC (pH 7), dehydrated in a cold ethanol series, and air dried.
The probes were denatured by heating at 75°C for 10 min, applied to chromosomal spreads (20 μL per slide), and incubated overnight in a moist chamber at 37°C. Posthybridization washes were performed at 43°C: twice in 50% (v/v) formamide/2X SSC, twice in 2X SSC, and once in 4X SSC-Tween-20, for 5 min each. Bound probe was detected by incubation with streptavidin-Cy3 (Amersham Biosciences). The chromosomes were counterstained in 0.3 μg/mL DAPI/2X SSC and mounted in a standard antifade solution (Vector).
2.4. Conventional Chromosome Banding
Conventional banding protocols were applied to the chromosomes of the two species differing from all the other probed species after FISH (the two Channichthyidae species, C. hamatus and P. macropterus) in order to gain structural details on the sex chromosomes. Chromosome spreads on microscope slides were DAPI (4,4′,6-diamidino-2-phenylindole) stained according to standard protocols. A characterization of the pattern of chromosome heterochromatin was performed through the C-banding method . Chromosome morphology was determined according to the centromere position following the nomenclature by Levan et al. .
2.5. Image Processing
Metaphase spreads were examined with an Olympus BX61 microscope equipped with a SenSys CCD camera (Photometrics). Photomicrographs were processed by CytoVision Genus software (Applied Imaging) and by the use of Adobe Photoshop software.
3.1. FISH Mapping of 5S rDNA and AFGPs Genes
Fluorescence in situ hybridization with a 5S rDNA probe resulted in a consistent single signal on a pair of submetacentric chromosomes in all trematomid species and in multiple signals onto the chromosomes of the two channichthyidae species (results summarized in Table 2). In one of the icefish species, C. hamatus, the probe hybridized on the long arm of the Y chromosome, at peritelomeric position (Figures 1(a) and 1(b)).
The AFGP probe hybridized at a single chromosomal position, on a pair of acrocentric chromosomes in the majority of the probed species. P. macropterus male differs from the others in showing hybridization signals at interstitial position on the Y chromosome and on a single acrocentric chromosome. Comparative analysis of the arms bearing the AFGP genes in P. macropterus showed that the signals on the Y chromosome and the acrocentric chromosome occupy a similar position with respect to the centromeres (Figures 1(c) and 1(d)).
3.2. Conventional Banding Analysis of C. hamatus and P. macropterus Chromosomes
Our analysis confirmed a diploid number in the female specimens, and in the males of both Channichthyidae species. The morphology of the male- and female-specific karyotypes in both the species has been previously reported . The odd diploid number in the males was always coupled with the presence of a multiple X1X2Y sex system (Figure 2).
C. hamatus male showed a sex linked heterochromosome system composed of a large submetacentric Y chromosome, a medium-sized submetacentric X1 chromosome, and an acrocentric X2 chromosome (shown enlarged in Figure 2(a)). X1 is unambiguously recognizable in the metaphase plates based on its morphology (submetacentric), size (medium sized), and banding pattern (homogeneously stained by DAPI); the X2 chromosome, similar to in morphology and size to several other acrocentrics in the karyotype, is identified by the subtelocentric position of 5S rDNA sequences after FISH mapping. The sex linked heterochromosome system of P. macropterus male consists of a large metacentric Y chromosome and two acrocentric chromosomes (X1 and X2) (shown enlarged in Figure 2(b)). In this species X1 and X2 were firstly assigned based on morphology (acrocentric) and size; in situ hybridization with an AFGP genes probe confirmed the occurrence of a large region of homology between the larger arm of Y chromosome and one acrocentric, thus allowing to assume the latter as the chromosome X2.
The C-banding revealed heterochromatic blocks in most of the centromeres on the chromosomes of both species (Figures 2(c) and 2(d)). In C. hamatus, heterochromatic blocks were also detected on some of the telomeres, on the small arm of a pair of chromosomes, and at pericentromeric position on at least four chromosomes. A C-band is present on the long arm of the Y chromosome at interstitial position (Figure 2(c)). In P. macropterus, heterochromatic blocks were mainly detected at centromeric and pericentromeric position; large heterochromatic bands were also found on the entire short arms of a pair of submetacentric chromosomes. Only centromeric constitutive heterochromatin was detected on the Y chromosome of this species (Figure 2(d)).
In fishes, sex chromosomes are not often recognizable, unless detailed genetic and FISH-based cytogenetic studies are applied . Among teleosteans, only a minority of species show heteromorphic sex-specific chromosomes [1, 10–13]. By contrast, the number of Antarctic notothenioid species possessing heteromorphic sex chromosomes is surprisingly high [14, 15].
In a longstanding cytogenetic screening effort of Notothenioidei, 45 notothenioid species living in the shelf waters of the High Antarctic Zoogeographic Zone and in the Southern part of the Seasonal Pack-ice Antarctic Zoogeographic Zone  have been cytogenetically studied. Out of those, 12 (26%) were found to have multiple sex chromosomes of the X1X1X2X2/X1X2Y type (Table 3).
The available data on the sex chromosomes in Antarctic notothenioid species has been previously described mainly through conventional cytogenetic techniques. At present, very little is known about their gene content [32–34], although this kind of structural cytogenomic information could allow to recognize the X1 and X2 chromosomes, thus providing clues for inferring the steps of the multiple sex chromosome system origin in the various species.
The herein described chromosomal location of 5S rDNA and AFGP genes on the Y-chromosomes of two channichthyidae species, C. hamatus and P. macropterus, respectively, contributes to improve the bulk of data on the gene content of sex chromosomes in Antarctic notothenioids and represents a starting point for reconstructing the chromosomal rearrangements that led to the formation of the secondary Y-chromosome in these two icefishes.
Hypothetical mechanisms of origin of the secondary Y-chromosome in Channichthyidae species had been suggested by Morescalchi et al. based on morphological traits . Although not providing the final proof, our findings are in agreement and provide support to previous hypotheses. The supposed mechanisms of origin of the sex chromosomes in the male of C. hamatus and P. macropterus are schematically illustrated in Figures 3(a) and 3(b), respectively.
In C. hamatus, the peritelomeric position of 5S rDNA repeats on the long arm of the Y chromosome, and at the same position in an acrocentric, supports the origin of the secondary-Y by tandem fusion of a submetacentric and an acrocentric chromosomes (Figure 3(a)). This is also supported by the localization of 5S rDNA sequences at peri-telomeric position on a pair of acrocentric chromosomes in the female (Figure 3(a′)), supposedly conserving the ancestral condition preceding the evolution of the heteromorphic sex chromosomes.
Similarly, the interstitial position of AFGP genes on an acrocentric chromosome and on the Y chromosome in P. macropterus male supports centric fusion between two acrocentrics of similar size as the most likely chromosomal rearrangement creating the secondary-Y in this species (schematized in Figure 3(b)). The chromosomal location of AFGP repeats in the same arm position on a pair of acrocentric chromosomes in the female (Figure 3(b′)) provides further support to this hypothesis.
Chromosomal rearrangements, that is, tandem or centric fusions, seem therefore to have been crucial events in the formation of the sex linked heterochromosomes in the two icefishes. This is in accordance with a recent hypothesis that postulates a major role of chromosomal rearrangements themselves for the origin of multiple sex chromosome systems against a diminished or immaterial role of heterochromatinization . Indeed, non significant amounts of constitutive heterochromatin have been detected through C-banding on the Y-chromosomes and/or on the X1 and X2 chromosomes of the two icefishes (Figures 2(c) and 2(d)). Notwithstanding, we cannot exclude that the presence of repetitive DNAs (5S rDNAs and AFGP sequences) onto the sex chromosomes of C. hamatus and P. macropterus would be a factor predisposing to further structural changes of the sex chromosomes. Indeed accumulation of repetitive sequences might occur, as the Y-chromosomes further evolve, as a consequence of the reduced rate of recombination between the sex chromosomes.
This first data on the gene content of the sex chromosomes in the two icefish species are relevant for tracing the architectural evolutionary changes of their Y-chromosomes. Moreover, the chromosomal location of 5S rDNA and AFGP genes provided a more general remarkable information: three patterns of association of these genes to the Y-chromosomes are recognizable in the eight Antarctic notothenioid species (see results summarized in Table 2). The distinctive differences revealed by the FISH analysis support the hypothesis that sex linked heterochromosomes likely arose independently in notothenioid fishes.
Interestingly, according to present information, heteromorphic sex chromosomes occur only in species of the Antarctic clade (families Artedidraconidae, Nototheniidae, Bathydraconidae, and Channichthyidae) (Figure 4), that experienced life in chronically cold marine waters over 10–14 million years. No morphologically differentiated sex chromosomes were ever found in species belonging to the non-Antarctic notothenioid families (Bovichtidae, Pseudaphritidae, and Eleginopidae) [36, 37], distributed in the sub-Antarctic region of the Southern Ocean, where they had not been exposed to natural selection by freezing marine conditions in their evolutionary history . The occurrence of heteromorphic sex chromosomes exclusively in Antarctic members of the Notothenioidei suborder might have interesting evolutionary/adaptive implications.
Considering the presence of cytogenetically distinct sex chromosomes as an evidence of a genetic control of sex determination stable for some time in a species [1, 7], the occurrence of sex heterochromosomes in Antarctic notothenioid species (and not in their non-Antarctic relatives), the high frequency of sex heterochromosomes in the Antarctic fish, and the independent origination of sex chromosomes in stenothermal notothenioids are suggestive of a convergent evolutionary trend towards a genetic sex determination system in Antarctic notothenioids. Indeed, the prominence of genetic control of sex determination seems adequate to assure proper sex ratio in an invariably cold and geographically isolated environment such as coastal Antarctic regions where these fishes live.
The current scanty information on the population genetics of notothenioid species prevents us from evaluating the influence of population genetic parameters that also might have played a role in the fixation of new chromosomal rearrangements leading to the formation of sex chromosomes.
A wider documentation on the frequency of sex linked chromosomes in a comprehensive sampling of Antarctic notothenioid taxa, the increase of population genetic data, and the use of new genomic tools that are being made available for Notothenioid fishes [19, 38, 39] will be the next steps to be walked on the way to investigate this intriguing evolutionary/adaptive hypothesis.
The study was supported by the Italian National Programme for Antarctic Research (PNRA) and contributes to the SCAR Scientific Research Program EBA (Evolution and Biodiversity in Antarctica). Part of L. Ghigliotti’s work was supported by the L’Oreal-UNESCO program “For Women in Science” through the 2008 National Fellowship “L’Oreal Italia Per le Donne e la Scienza.” C-HCC acknowledges NSF OPP for grant award that supported parts of this study. The authors gratefully acknowledge Catherine Ozouf-Costaz (Département Systématique et Evolution, MNHN Paris) for her comments and supervision of the work at the MNHN.
- R. H. Devlin and Y. Nagahama, “Sex determination and sex differentiation in fish: an overview of genetic, physiological, and environmental influences,” Aquaculture, vol. 208, no. 3-4, pp. 191–364, 2002.
- J. E. Mank, D. E. L. Promislow, and J. C. Avise, “Evolution of alternative sex-determining mechanisms in teleost fishes,” Biological Journal of the Linnean Society, vol. 87, no. 1, pp. 83–93, 2006.
- D. K. Griffin, S. C. Harvey, R. Campos-Ramos et al., “Early origins of the X and Y chromosomes: lessons from tilapia,” Cytogenetic and Genome Research, vol. 99, no. 1–4, pp. 157–163, 2002.
- M. Schartl, “Sex chromosome evolution in non-mammalian vertebrates,” Current Opinion in Genetics and Development, vol. 14, no. 6, pp. 634–641, 2004.
- D. Charlesworth, B. Charlesworth, and G. Marais, “Steps in the evolution of heteromorphic sex chromosomes,” Heredity, vol. 95, no. 2, pp. 118–128, 2005.
- R. Ming and P. H. Moore, “Genomics of sex chromosomes,” Current Opinion in Plant Biology, vol. 10, no. 2, pp. 123–130, 2007.
- N. Ospina-Álvarez and F. Piferrer, “Temperature-dependent sex determination in fish revisited: prevalence, a single sex ratio response pattern, and possible effects of climate change,” PLoS One, vol. 3, no. 7, Article ID e2837, 2008.
- J. A. Ross, J. R. Urton, J. Boland, M. D. Shapiro, and C. L. Peichel, “Turnover of sex chromosomes in the stickleback fishes (Gasterosteidae),” PLoS Genetics, vol. 5, no. 2, Article ID e1000391, 2009.
- T. J. Near, J. J. Pesavento, and C.-H. C. Cheng, “Phylogenetic investigations of Antarctic notothenioid fishes (Perciformes: Notothenioidei) using complete gene sequences of the mitochondrial encoded 16S rRNA,” Molecular Phylogenetics and Evolution, vol. 32, no. 3, pp. 881–891, 2004.
- J. E. Mank and J. C. Avise, “Evolutionary diversity and turn-over of sex determination in teleost fishes,” Sexual Development, vol. 3, no. 2-3, pp. 60–67, 2009.
- A. J. Solari, Sex Chromosomes and Sex Determination in Vertebrates, Taylor & Francis, Boca Raton, Fla, USA, 1994.
- M. Klinkhartdt, M. Tesche, and H. Greven, Database of Fish Chromosomes, Westarp Wissenschaften, Magdeburg, Germany, 1995.
- R. Arai, Fish Karyotypes: A Checklist, Springer, Tokyo, Japan, 2011.
- E. Pisano and C. Ozouf-Costaz, “Cytogenetics and evolution in extreme environment: the case of Antarctic fishes,” in Fish Adaptations, A. Val and G. Kapoor, Eds., pp. 311–338, Oxford 6 IBH Publishing, New Delhi, India, 2003.
- L. Ghigliotti, T. J. Near, S. Ferrando, M. Vacchi, and E. Pisano, “Cytogenetic diversity in the Antarctic plunderfishes (Notothenioidei: Artedidraconidae),” Antarctic Science, vol. 22, no. 6, pp. 805–814, 2010.
- J. T. Eastman, “The nature of the diversity of Antarctic fishes,” Polar Biology, vol. 28, no. 2, pp. 93–107, 2005.
- C.-H. C. Cheng and H. W. Detrich, “Molecular ecophysiology of Antarctic notothenioid fishes,” Philosophical Transactions of the Royal Society B, vol. 362, no. 1488, pp. 2215–2232, 2007.
- Z. L. Coppes Petricorena and G. N. Somero, “Biochemical adaptations of notothenioid fishes: Comparisons between cold temperate South American and New Zealand species and Antarctic species,” Comparative Biochemistry and Physiology—A Molecular and Integrative Physiology, vol. 147, no. 3, pp. 799–807, 2007.
- Z. Chen, C.-H. C. Cheng, J. Zhang et al., “Transcriptomic and genomic evolution under constant cold in Antarctic notothenioid fish,” Proceedings of the National Academy of Sciences of the United States of America, vol. 105, no. 35, pp. 12944–12949, 2008.
- A. L. DeVries, “Glycoproteins as biological antifreeze agents in Antarctic fishes,” Science, vol. 172, no. 3988, pp. 1152–1155, 1971.
- A. L. de Vries and C.-H. C. Cheng, “Antifreeze proteins and organismal freezing avoidance in polar fishes,” in The Physiology of Polar Fishes, A. P. Farrell and J. F. Steffenson, Eds., pp. 155–201, Elsevier, San Diego, Calif, USA, 2005.
- J. Nicodemus-Johnson, S. Silic, L. Ghigliotti, E. Pisano, and C.-H. C. Cheng, “Assembly of the antifreeze glycoprotein/trypsinogen-like protease genomic locus in the Antarctic fish Dissostichus mawsoni (Norman),” Genomics, vol. 98, no. 3, pp. 194–201, 2011.
- L. Ghigliotti, S.-E. Fevolden, C.-H. C. Cheng, I. Babiak, A. Dettai, and E. Pisano, “Karyotyping and cytogenetic mapping of Atlantic cod (Gadus morhua Linnaeus, 1758),” Animal Genetics, vol. 43, no. 6, pp. 746–752, 2012.
- A. Morescalchi, E. Pisano, R. Stanyon, and M. A. Morescalchi, “Cytotaxonomy of antarctic teleosts of the Pagothenia/Trematomus complex (Nototheniidae, Perciformes),” Polar Biology, vol. 12, no. 6-7, pp. 553–558, 1992.
- C. Ozouf-Costaz, J. C. Hureau, and M. Beaunier, “Chromosome studies on fish of the suborder Notothenioidei collected in the Weddell Sea during EPOS 3 cruise,” Cybium, vol. 15, no. 4, pp. 271–289, 1991.
- C. Ozouf-Costaz, “Karyotypes of Chaenodraco wilsoni and Chionodraco myersi (Channichthyidae) from Prydz Bay, Antarctica,” Copeia, vol. 1987, pp. 503–505, 1987.
- A. Morescalchi, J. C. Hureau, E. Olmo, C. Ozouf-Costaz, E. Pisano, and R. Stanyon, “A multiple sex-chromosome system in Antarctic ice-fishes,” Polar Biology, vol. 11, no. 8, pp. 655–661, 1992.
- A. T. Sumner, “A simple technique for demonstrating centromeric heterochromatin,” Experimental Cell Research, vol. 75, no. 1, pp. 304–306, 1972.
- A. Levan, K. Fredga, and A. A. Sandberg, “Nomenclature for centromeric position on chromosome,” Hereditas, vol. 52, pp. 201–220, 1964.
- J. A. Ross and C. L. Peichel, “Molecular cytogenetic evidence of rearrangements on the Y chromosome of the threespine stickleback fish,” Genetics, vol. 179, no. 4, pp. 2173–2182, 2008.
- K. H. Kock, Antarctic Fish and Fisheries, Cambridge University Press, Cambridge, UK, 1992.
- T. Capriglione, G. Odierna, V. Caputo, A. Canapa, and E. Olmo, “Characterization of a Tc1-like transposon in the antarctic ice-fish, Chionodraco hamatus,” Gene, vol. 295, no. 2, pp. 193–198, 2002.
- C. Ozouf-Costaz, J. Brandt, C. Körting et al., “Genome dynamics and chromosomal localization of the non-LTR retrotransposons Rex1 and Rex3 in Antarctic fish,” Antarctic Science, vol. 16, no. 1, pp. 51–57, 2004.
- F. Mazzei, L. Ghigliotti, C. Bonillo, J.-P. Coutanceau, C. Ozouf-Costaz, and E. Pisano, “Chromosomal patterns of major and 5S ribosomal DNA in six icefish species (Perciformes, Notothenioidei, Channichthyidae),” Polar Biology, vol. 28, no. 1, pp. 47–55, 2004.
- M. B. Cioffi, O. Moreira-Filho, L. F. Almeida-Toledo, and L. A. C. Bertollo, “The contrasting role of heterochromatin in the differentiation of sex chromosomes: an overview from Neotropical fishes,” Journal of Fish Biology, vol. 80, no. 6, pp. 2125–2139, 2012.
- F. Mazzei, L. Ghigliotti, G. Lecointre et al., “Karyotypes of basal lineages in notothenioid fishes: the genus Bovichtus,” Polar Biology, vol. 29, no. 12, pp. 1071–1076, 2006.
- F. Mazzei, L. Ghigliotti, J. P. Coutanceau et al., “Chromosomal characteristics of the temperate notothenioid fish Eleginops maclovinus (Cuvier),” Polar Biology, vol. 31, no. 5, pp. 629–634, 2008.
- H. W. Detrich and C. T. Amemiya, “Antarctic notothenioid fishes: genomic resources and strategies for analyzing an adaptive radiation,” Integrative and Comparative Biology, vol. 50, no. 6, pp. 1009–1017, 2010.
- S. C. Shin, S. J. Kim, J. K. Lee et al., “Transcriptomics and comparative analysis of three Antarctic Notothenioid fishes,” PLoS One, vol. 7, no. 8, Article ID e43762, 2012.
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