Research Article
Polymorphism of the Transferrin Gene in Eye Diseases: Keratoconus and Fuchs Endothelial Corneal Dystrophy
Table 2
Data for PCR-RFLP analysis used to genotype polymorphisms in the TF gene.
| Polymorphism | Primers sequences (forward, reverse) | Annealing temperatures in PCR (°C) | Restriction enzymes (recognized allele) | Fragments (bp) |
| g.3296G>A rs8177178 | AGGGCATAGAGCTGGCTGCT GAAGACTGTTAGCATGAGGGCC | 66.2 | RsaI (A) | GG 483 GA 483/327/156 AA 327/156 | g.3481A>G rs8177179 | AGCTGTATGTGTGCATGCTGCTC GGGCCAATTCACACATTCAAT | 60.5 | StyI (A) | AA 274/198 AG 472/274/198 GG 472 | c.–2G>A rs1130459 | AGAAAATGAGGTGATCAGTGGG ATGGAAAGGCACCCAGACAC | 66.0 | FokI (G) | GG 218/157 GA 375/218/157 AA 375 |
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