Clinical Study

A Flow Cytometric Analysis of Vitreous Inflammatory Cells in Patients with Proliferative Diabetic Retinopathy

Table 1

Antibodies for 8-colour immunophenotyping of vitreous and peripheral venous blood samples.

Test tubeFITCPEPerCP-Cy5.5APCPE-Cy7APC-Cy7

1Kappa*  + CD4*Lambda*  + CD8*CD45*CD14**CD19**CD3**

Legend: mAbs: monoclonal antibodies, FITC: fluorescein isothiocyanate, PE: phycoerythrin, PerCP-Cy5.5: peridinin chlorophyll protein-Cy5.5, APC: allophycocyanin, PE-Cy7: phycoerythrin-cyanin 7, APC-Cy7: allophycocyanin-cyanin 7; *5 μL monoclonal antibody was added; **3 μL monoclonal antibody was added.