The Role of Bone Marrow Mesenchymal Stem Cells in the Treatment of Acute Liver Failure
Figure 5
Immunofluorescence detection. The samples were prepared according to the protocol. VEGF antibody and the AFP antibody were used. FITC-labeled secondary antibody was added and the solution was incubated for 15 minutes. Then, DAPI was used to stain nucleus. The cells were observed under fluorescence microscope. The numbers of fluorescent-labeled liver cells and the total numbers of liver cells in each field were counted. (a) VEGF protein expression in liver tissues. (b) AFP protein expression in liver tissues.
