Mesenchymal Stem Cell-Like Cells Derived from Mouse Induced Pluripotent Stem Cells Ameliorate Diabetic Polyneuropathy in Mice

Figure 1

Senescence-associated beta-galactosidase (SA β-Gal) staining. (a) The induced pluripotent stem cell colonies obtained from aged mice colonies were heterogeneously expressed with SA β-Gal (green). Scale bar = 50 μm. (b) After long term culture, differentiated MSC-like cells expressing SA β-Gal (green) decreased. Scale bar = 100 μm. (c) The ratio of SA β-Gal positive mesenchymal stem cell (MSC)-like cells decreased significantly in 10 weeks or 16 weeks cultured MSC-like cells compared with 4 weeks cultured cells. 4 wks: the MSC-like cells cultured for 4 weeks, 10 wks: the MSC-like cells cultured for 10 weeks, and 16 wks: the MSC-like cells cultured for 16 weeks. ^∗∗𝑃<0.005 compared with 4 wks.

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