Mesenchymal Stem Cell-Like Cells Derived from Mouse Induced Pluripotent Stem Cells Ameliorate Diabetic Polyneuropathy in Mice
Figure 3
The characterization of the mesenchymal stem cell (MSC)-like cells. (a) Flow cytometry analyses of mesenchymal stem cell (MSC) and hematopoietic cell lineage markers in the MSC-like cells. The MSC-like cells expressed MSC markers, CD105, CD140a, Sca-1, and CD44, in spite of no expression of hematopoietic markers, CD34, TER119, CD31, CD45, and CD11b. Open curves: control, filled curves: each of target antibodies. (b) After differentiation of the MSC-like cells in vitro, each differentiated cells into chondrocyte, osteoblast, and adipocyte exhibited staining abilities with Alcian blue, alkaline phosphatase, and Oil Red-O, respectively. ALP: alkaline phosphatase staining, scale bar = 50 μm.
