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BioMed Research International
Volume 2013, Article ID 263509, 10 pages
Research Article

Antioxidant Capacity and Antimutagenic Potential of Murraya koenigii

1Department of Agricultural Microbiology, Aligarh Muslim University, Aligarh 202 002, India
2James Graham Brown Cancer Center, University of Louisville, Louisville, KY 40202, USA

Received 11 April 2013; Accepted 2 June 2013

Academic Editor: Vincent Ribrag

Copyright © 2013 Maryam Zahin et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Gas Chromatography Mass Spectrometery (GC-MS): The benzene fraction of the extracts was subjected to GC-MS in order to identify the active constituents. The GC was done on GCD 1800 A, Hewlett Packard, coupled with HP-1 column (30 m × 0.25 mm × 0.25 μm; Thermo Scientific, USA). Injector and detector temperatures were 250°C and 280°C, respectively. The carrier gas used was helium at 1 mL/min; initial temperature of oven was 100–250°C at the rate of 10°C/min, hold time was at 250°C for 3 min and final temperature was 250–280°C at the rate of 30°C/min and hold time at 280°C for 2 min. The solvent used for making all dilutions was methanol.

A total of 21 chemical components were identified in leaf extract by GC-MS analysis (Figure S1). These numbers may be extended with the help of chemo metric techniques. The major compounds identified were caryophyllene (14.8%) followed by 3-undecen-5-yne (Z)-(9.52%), phytol (9.17%), 2-methyl-3H-phenanthro[3,4-D] imida (8.90%), caryophyllene oxide (6.61%), propylparaben (6.11%), D-limonene (6.01%). The remaining compounds were present in percentages of (1.06-5.72) as depicted in Figure S2, S3 and S4 and table S1. Figures S1-S4 demonstrate the mass spectrometric analysis of major 12 peaks demonstrated in Table S1.

  1. Suplementary Materials