Development of a Broad-Range 23S rDNA Real-Time PCR Assay for the Detection and Quantification of Pathogenic Bacteria in Human Whole Blood and Plasma Specimens
Figure 3
Analytical sensitivities of three different extraction protocols following quantification by the novel 23S rDNA real-time PCR from whole blood (continuous line) and PBS (dotted line) spiked with different Gram-negative (Escherichia coli) or Gram-positive (Staphylococcus aureus) bacteria ranging from 1 to 105 bacteria per reaction. EasyMag automated extraction protocols with E. coli (a) or with S. aureus (d) spiked in PBS and in whole blood. QIAmp DNA blood mini kit extraction methods with E. coli (b) or with S. aureus (e) spiked in PBS and in whole blood; QIAmp DNA blood mini kit extraction with pretreatment with lysozyme and proteinase K with E. coli (c) or with S. aureus (f), spiked in PBS and in whole blood. Results are representative of three independent experiments, effected on three independent DNA extractions and expressed as mean ± standard deviations.
