Development of a Broad-Range 23S rDNA Real-Time PCR Assay for the Detection and Quantification of Pathogenic Bacteria in Human Whole Blood and Plasma Specimens

Table 1

Bacterial strains utilized in this study. The strains were either obtained from the bacterial collection of the St. Orsola Hospital (BACSO) or derived from routine workflow. In this last case the procedure for identification are reported in the Qualty Assurance files of the Laboratory.

Microorganisms species	Origin of the isolate
Acinetobacter baumannii	Urine
Acinetobacter lwoffii	Urine
Alcaligenes xylosoxidans	Urine
Bacteroides fragilis	Cerebrospinal fluid
Campylobacter jejuni	Feces
Citrobacter braakii	Abdominal drainage
Citrobacter freundii	Blood
Citrobacter koseri	Urine
Corynebacterium jeikeium	Blood
Corynebacterium minutissimum	Blood
Corynebacterium striatum	Blood
Corynebacterium urealyticum	Blood
Enterobacter cloacae	Urine
Enterobacter aerogene	Urine
Enterococcus casseliflavus	Blood
Enterococcus faecalis	Bacso/atcc 29212
Enterococcus faecium	Blood
Enterococcus gallina rum	Blood
Escherichia coli	Bacso/atcc 25922
Haemophilus influenzae	Bacso/atcc 49247
Haemophilus influenzae	Bacso/atcc 49766
Hafnia alvei	Bile
Klebsiella oxytoca	Blood
Klebsiella pneumoniae	Urine
Morganella morganii	Urine
Nocardia sp.	Bronchial aspirate
Proteus mirabilis	Urine
Proteus vulgaris	Bronchial aspirate
Providencia stuartii	Urine
Pseudomonas aeruginosa	Bacso/atcc 27853
Pseudomonas luteola	Bronchial aspirate
Salmonella sp. Group B	Feces
Salmonella sp. Group C	Feces
Salmonella sp. Group D	Feces
Serratia marcescens	Urine
Staphylococcus aureus	Bacsoatcc 29213
Staphylococcus epidermidis	Blood
Staphylococcus haemolyticus	Blood
Staphylococcus hominis	Blood
Staphylococcus warneri	Blood
Stenotrophomonas maltophilia	Faringeal swab
Streptococcus agalactiae	Uretral swab
Streptococcus anginosus	Blood
Streptococcus mitis	Blood
Streptococcus parasanguinis	Blood
Streptococcus pyogene	Faringeal swab
Streptococcus pneumoniae	Bacso/atcc 49619