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BioMed Research International
Volume 2013, Article ID 268249, 10 pages
Research Article

Constitutive Optimized Production of Streptokinase in Saccharomyces cerevisiae Utilizing Glyceraldehyde 3-Phosphate Dehydrogenase Promoter of Pichia pastoris

1Centre for Biotechnology, Institute of Science and Technology, Jawaharlal Nehru Technological University, Kukatpally, Hyderabad, Andhra Pradesh 500085, India
2Ontario Cancer Institute, Princess Margaret Cancer Centre, 610 University Avenue, Toronto, ON, Canada M5G 2M9
3Department of Chemical Engineering, Monash University, Clayton, VIC 3800, Australia

Received 25 April 2013; Revised 27 June 2013; Accepted 10 July 2013

Academic Editor: Gotthard Kunze

Copyright © 2013 Ravi N. Vellanki et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Supplementary Material

Tables S1 to S6 are statistical information used and generated while optimizing process conditions for better SK production by Saccharomyces cerevisiae. Table S1 and S2 are the design matrix for screening carbon and organic nitrogen sources by using Placket-Burmun design. Experiments were conducted according to the design matrix and results are used in subsequent optimization studies. Table S3 is the range and levels of four variables considered for optimization. Table S4 is the design matrix generated according to central composite design for response surface methodology. Table S5 and S6 are ANOVA of quadratic equation and responses multiple linear regression analysis respectively.

  1. Supplementary Tables