Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits

<div class="floats-full-page floats-full-page-other"><h2>Table 1</h2><div class="floats-full-page-caption">Compounds dependent MS/MS parameters for Chromsystems (a) and for PerkinElmer (b) assays.</div><div class="floats-full-page-content"><table class="table-group" id="tab1"><tr class="table-caption" id="a"><td><b>(a) </b></td></tr><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr /></td></tr><tr class="thead"><td align="center" colspan="4">Chromsystems</td></tr><tr class="thead"><td align="left">Compound</td><td align="center">SRM</td><td align="center">Collision Energy [eV]</td><td align="center">Tube Lens [V]</td></tr><tr><td class="thead-hr" colspan="4"><hr /></td></tr><tr><td align="left">25(OH)D<sub>3</sub></td><td align="center">383.2 <svg height="7.6374998" id="M4" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 210.8</td><td align="center">23</td><td align="center">80</td></tr><tr><td align="left">25(OH)D<sub>2</sub></td><td align="center">395.2 <svg height="7.6374998" id="M5" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 268.8</td><td align="center">17</td><td align="center">80</td></tr><tr><td align="left">25(OH)D<sub>3</sub>/D<sub>2</sub> IS</td><td align="center">389.3 <svg height="7.6374998" id="M6" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 210.8</td><td align="center">28</td><td align="center">80</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr" /></td></tr></table></td></tr><tr class="table-caption" id="b"><td><b>(b) </b></td></tr><tr><td><table class="table"><tr><td class="thead-hr" colspan="4"><hr /></td></tr><tr class="thead"><td align="center" colspan="4">PerkinElmer</td></tr><tr class="thead"><td align="left">Compound</td><td align="center">SRM</td><td align="center">Collision Energy [eV]</td><td align="center">Tube Lens [V]</td></tr><tr><td class="thead-hr" colspan="4"><hr /></td></tr><tr><td align="left">25(OH)D<sub>3</sub> Cal</td><td align="center">613.4 <svg height="7.6374998" id="M7" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 298.1</td><td align="center">19</td><td align="center">110</td></tr><tr><td align="left">25(OH)D<sub>3</sub> IS</td><td align="center">610.1 <svg height="7.6374998" id="M8" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 310.1</td><td align="center">19</td><td align="center">110</td></tr><tr><td align="left">25(OH)D<sub>3</sub></td><td align="center">607.4 <svg height="7.6374998" id="M9" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 298.1</td><td align="center">19</td><td align="center">110</td></tr><tr><td align="left">25(OH)D<sub>2</sub> Cal</td><td align="center">625.4 <svg height="7.6374998" id="M10" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 298.1</td><td align="center">17</td><td align="center">110</td></tr><tr><td align="left">25(OH)D<sub>2</sub> IS</td><td align="center">622.4 <svg height="7.6374998" id="M11" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 301.1</td><td align="center">17</td><td align="center">110</td></tr><tr><td align="left">25(OH)D<sub>2</sub></td><td align="center">619.4 <svg height="7.6374998" id="M12" style="vertical-align:-0.0pt" version="1.1" viewbox="0 0 22.012501 7.6374998" width="22.012501" xmlns="http://www.w3.org/2000/svg" xmlns:xlink="http://www.w3.org/1999/xlink">
<g transform="matrix(.017,-0,0,-.017,2.9,7.575)"><path d="M901 255q-71 -62 -185 -187l-22 15l102 147h-727v50h727l-102 147l22 15q114 -125 185 -187z" id="x2192"></path></g>
</svg> 298.1</td><td align="center">17</td><td align="center">110</td></tr><tr class="table-tr"><td colspan="4"><hr class="tbody-hr" /></td></tr></table></td></tr></table></div></div>

Table 1 | Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits

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Table 1: Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits

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Table 1

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