BioMed Research International / 2013 / Article / Tab 1

Methodology Report

Total 25-Hydroxyvitamin D Determination by an Entry Level Triple Quadrupole Instrument: Comparison between Two Commercial Kits

Table 1

Compounds dependent MS/MS parameters for Chromsystems (a) and for PerkinElmer (b) assays.
(a)

Chromsystems
CompoundSRMCollision Energy [eV]Tube Lens [V]

25(OH)D3383.2 210.82380
25(OH)D2395.2 268.81780
25(OH)D3/D2 IS389.3 210.82880

(b)

PerkinElmer
CompoundSRMCollision Energy [eV]Tube Lens [V]

25(OH)D3 Cal613.4 298.119110
25(OH)D3 IS610.1 310.119110
25(OH)D3607.4 298.119110
25(OH)D2 Cal625.4 298.117110
25(OH)D2 IS622.4 301.117110
25(OH)D2619.4 298.117110