NSOM/QD-Based Visualization of GM1 Serving as Platforms for TCR/CD3 Mediated T-Cell Activation

Figure 3

NSOM/QD-based dual-color imaging of CD3 and GM1 for T-cells costimulated with anti-CD3/anti-CD28 Ab. (a) T-cell topography, (b) fluorescence image of QD-bound CD3 (red). (c) Fluorescence image of QD-bound GM1 (blue). (d) Two color fluorescence merge image. (e) Topography-fluorescence merge image. ((f), (g), and (h)) Zoom images of the areas as indicated by the squares on (b), (c) and (d), respectively. (i) The percentage numbers of TCR/CD3 or GM1 molecules that are arrayed to form nanodomains. (j) Molecule density of TCR/CD3 nanodomains or GM1 nanodomains. Scale bars are 1 μm for (a), (b), (c), (d) and (e) and 200 nm for (f), (g) and (h).

(a)

(b)

(c)

(d)

(e)

(f)

(g)

(h)

(i)

(j)