BioMed Research International / 2013 / Article / Fig 1

Research Article

Frataxin mRNA Isoforms in FRDA Patients and Normal Subjects: Effect of Tocotrienol Supplementation

Figure 1

(a) A representative scheme of frataxin isoform cDNAs and primer sequences. Frataxin isoform 1 (FXN-1) and isoform 2 (FXN-2) share the left primer sequence, while the right primer was specific for each isoform. Frataxin isoform 3 (FXN-3) right primer sequence was designed in the region downstream the 702 base pair. (b) Enzymatic digestion of FXN-1 and FXN-2 PCR products. A representative polyacrylamide gel electrophoresis (10%) of FXN-1 and FXN-2 PCR products digested or undigested with BstNI restriction enzyme. Lane 1: DNA ladder; lane 2: undigested FXN-1 PCR product; Lane 3: BstNI digested FXN-1 PCR product; lane 4: undigested FXN-2 PCR product; lane 5: BstNI digested-FXN-2 PCR product.
(a) Frataxin isoform cDNAs and primer sequences
(b) Enzymatic digestion of FXN-1 and FXN-2 PCR products

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