Research Article
Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease
Table 3
ANOVA for the response surface quadratic polynomial model.
| Source | Sum of squares | df | Mean square | value | value | Significance |
| Model | 0.03 | 14 | | 20.16 | <0.00 | **a | —pH | | 1 | | 1.11 | 0.39 | | —temperature | | 1 | | 70.40 | <0.00 | ** | —E/S | | 1 | | 48.84 | <0.00 | ** | —incubation period | | 1 | | 102.04 | <0.00 | ** | | | 1 | | 0.43 | 0.52 | | | | 1 | | 2.34 | 0.15 | | | | 1 | | 1.15 | 0.30 | | | | 1 | | 0.81 | 0.38 | | | | 1 | | 1.86 | 0.19 | | | | 1 | | 7.79 | 0.01 | ****c | | | 1 | | 2.62 | 0.13 | | | | 1 | | 3.62 | 0.08 | | | | 1 | | 12.32 | 0.00 | ***b | | | 1 | | 22.72 | 0.00 | ** | Residual | | 15 | | | | | Lack of fit | | 10 | | 0.43 | 0.88 | | Pure error | | 5 | | | | | Cor. total | 0.03 | 29 | | | | |
| Std. Dev. | | | | -squared | 0.95 | | Mean |
0.29
| | | Adj. -squared | 0.90 | | C.V.% | | | | Pred. -squared | 0.83 | | PRESS | | | | Adeq. precisior | 18.81 | |
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df: degree of freedom.
aSignificance at 0.001 level.
bSignificance at 0.01 level.
cSignificance at 0.05 level
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