Optimisation of the Enzymatic Hydrolysis of Blood Cells with a Neutral Protease

Table 4

Free amino acid and total amino acid composition of BCs and EHBCs (g/100 g) and the EAA score compared with the FAO/WHO reference protein.

	Free amino acid		Total amino acid
Amino Acids	BCs	EHBCs	BCs	EHBCs
Lysine	—	4.26±0.05	8.16±0.10	5.07±0.08
Methionine	0.19±0.00	1.09±0.06	0.77±0.05	0.49±0.02
Threonine	0.03±0.00	1.28±0.06	3.00±0.05	1.92±0.02
Arginine	—	3.08±0.04	4.21±0.11	2.79±0.06
Histidine	—	2.84±0.05	7.21±0.09	4.12±0.06
Isoleucine	—	—	0.19±0.01	0.21±0.01
Leucine	—	6.52±0.05	15.49±0.20	9.90±0.08
Phenylalanine	0.06±0.00	4.48±0.04	6.68±0.17	4.46±0.06
Valine	—	1.38±0.06	7.45±0.25	4.97±0.05
Tryptophan	—	—	—	—
Serine	0.03±0.00	0.56±0.03	4.30±0.12	2.33±0.07
Glutamic acid	0.08±0.00	1.36±0.04	8.06±0.10	6.03±0.05
Glycine	0.33±0.01	1.42±0.04	4.52±0.10	3.25±0.05
Proline	—	1.18±0.03	3.17±0.09	2.27±0.07
Cystine	0.11±0.00	0.25±0.01	0.31±0.03	0.15±0.01
Aspartic acid	0.02±0.00	1.34±0.02	11.32±0.54	7.81±0.09
Alanine	—	2.99±0.04	7.73±0.08	5.46±0.06
Tyrosine	—	1.21±0.01	1.35±0.05	0.85±0.05
Total	0.85±0.02	35.24±0.13	93.91±0.06	62.08±0.05
Amino acid	EAA Score
	Reference; protein 1^a	Reference; protein 2^b	RP1^c		RP2^d
			BCs	EHBCs	BCs	EHBCs
Lysine	5.80	5.25	1.41±0.02	0.87±0.02	1.55±0.02	0.97±0.02
Threonine	3.40	3.90	0.88±0.02	0.56±0.01	0.77±0.01	0.49±0.01
Valine	3.50	3.60	2.13±0.07	1.42±0.01	2.07±0.07	1.38±0.01
Isoleucine	2.80	2.90	0.07±0.00	0.08±0.01	0.07±0.01	0.07±0.01
Leucine	6.60	5.20	2.35±0.03	1.50±0.01	2.98±0.04	1.90±0.02
Phenylalanine and tyrosine	6.30	4.85	1.27±0.04	0.84±0.00	1.66±0.05	1.09±0.01
Tryptophan	1.10	0.95	—	—	—	—
Methionine and cystine	2.50	3.00	0.43±0.03	0.26±0.02	0.36±0.03	0.21±0.01
Histidine	1.90	1.65	3.79±0.05	2.17±0.03	4.37±0.05	2.50±0.04
Arginine	—	2.10	—	—	2.00±0.06	1.33±0.03

^aSuggested profile of essential amino acid requirements for adults (FAO/WHO, 1991) [27].
^bEssential amino acid requirements of the common pig (3–10 kg) according to the NRC (1998) [28].
^cChemical score calculated using the FAO/WHO reference protein as the base.
^dChemical score calculated using the amino acid requirements as per the NRC (1998) [28].