Secretion of Biologically Active Heterologous Oxalate Decarboxylase (OxdC) in Lactobacillus plantarum WCFS1 Using Homologous Signal Peptides
Figure 6
Oxalate degradation capabilities of wild type and recombinant L. plantarum WCFS1. (a) Oxalate degradation ability of wild type L. plantarum WCFS1 corresponding simples of WCFS1 mM oxalate degradation and growth curve indicate concentration of oxalate reduction in supernatant against the growth of the wild type L. plantarum WCFS1 strain, respectively; (b) oxalate degradation capability of recombinant strains corresponding simples of 3050 WCFS1, 0373 WCFS1 mM oxalate degradation and growth curve indicate concentration of oxalate reduction in supernatant of recombinant L. plantarum WCFS1 strains harboring plasmid pLp_3050OxdC and pLp_0373OxdC, respectively, against the growth of the strains. Arrow indicates the point of induction using peptide (sppIP) at OD600 ~ 0.3.
