Secretion of Biologically Active Heterologous Oxalate Decarboxylase (OxdC) in Lactobacillus plantarum WCFS1 Using Homologous Signal Peptides

Table 2

Primers and induction peptide used in this study.

Primers/peptide	Sequences (5′→3′)^a
OXDC sense	5′-GAGAGTCGACATGAAAAAACAAAATGACATTCCGC-3′ (Sal I)
OXDC antisense	5′-GGAATTCGTGGTGGTGGTGGTGGTGTTATTTACTGCATTTCTTTTTCACTA-3′ (EcoR I)
PsppA sense	5′-GTCACTAACCTGCCCCGTTA-3′
SppIP	MAGNSSNFIHKIKQIFTHR*

aThe restriction sites are underlined;∗aminoacid sequences of induction peptide (SppIP).