283856.fig.003a
(a)
283856.fig.003b
(b)
283856.fig.003c
(c)
283856.fig.003d
(d)
283856.fig.003e
(e)
Figure 3: Representative Western blots for MMP9, MMP2, NGAL, and TIMP1 detection in the porcine homogenates of LV myocardium performed in reducing and nondenaturing conditions (a 5-minute incubation in 0.1 M dithiothreitol [DTT] at a room temperature) (a) and in reducing and denaturing conditions (a 5-minute incubation in 0.1 M DTT at 95°C) (c). Positive controls for reducing and nondenaturing conditions (b) and reducing and denaturing conditions (d). Representative Western blots in the porcine homogenates of LV myocardium from a healthy pig and animals with mild, moderate, and severe HF with anti-NGAL antibodies in reducing and denaturing conditions (e).