Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies

Figure 4

Immunoblot inhibition. B. alba native extract (65 μg/line) in solid phase. Line 1: Rabbit 978 (immunized with native extract), line 2: Rabbit 979 (immunized with native extract), line 3: Rabbit 976 (immunized with Dpg-Pol extract), and line 4: Rabbit 977 (immunized with Dpg-Pol extract); lines 1 to 4 were inhibited with native extract (650 μg of B. alba native extract). Line 5: Rabbit 979 without inhibition, line 6: Rabbit 979 inhibited with BSA, and line 7: negative control (PBST+5% milk). Sera were used at a dilution 1 : 400.