Depigmented Allergoids Reveal New Epitopes with Capacity to Induce IgG Blocking Antibodies

Table 1

Synthetic peptides of Bet v 1 and Bet v 2. They are synthetized overlapping by 6 aminoacids; their position in the sequence, and the recognition by the IgG induced after the immunization with Dpg-Pol (D-P) or native (N) extracts are shown.

Number	Sequence	Position	IgG extract recognition
Bet v 1
1	MGVFNYETETTS	1–12
2	ETETTSVIPAAR	7–18	D-P/N
3	VIPAARLFKAFI	13–24	D-P/N
4	LFKAFILDGDNL	19–30	N
5	LDGDNLFPKVAP	25–36
6	FPKVAPQAISSV	31–42	D-P/N
7	QAISSVENIEGN	37–48	D-P
8	ENIEGNGGPGTI	43–54	D-P
9	GGPGTIKKISFP	49–60	N
10	KKISFPEGFPFK	55–66	N
11	EGFPFKYVKDRV	61–72
12	YVKDRVDEVDHT	67–78
13	DEVDHTNFKYNY	73–84
14	NFKYNYSVIEGG	79–90
15	SVIEGGPIGDTL	85–96	D-P/N
16	PIGDTLEKISNE	91–102	N
17	EKISNEIKIVAT	97–108	N
18	IKIVATPDGGSI	103–114	D-P
19	PDGGSILKISNK	109–120	D-P/N
20	LKISNKYHTKGD	115–126
21	YHTKGDHEVKAE	121–132
22	HEVKAEQVKASK	127–138	N
23	QVKASKEMGETL	133–144
24	EMGETLLRAVES	139–150
25	LRAVESYLLAHS	145–156
26	YLLAHSDAYN	151–160
Bet v 2
1	MSWQTYVDEHLM	1–12
2	YVDEHLMCDIDG	6–17
3	LMCDIDGQASNS	11–22
4	DGQASNSLASAI	16–27
5	SLASAIVGHDGS	22–33
6	AIVGHDGSVWAQ	26–37
7	DGSVWAQSSSFP	31–42
8	AQSSSFPQFKPQ	36–47	D-P
9	FPQFKPQEITGI	41–52	D-P/N
10	PQEITGIMKDFE	46–57	D-P/N
11	GIMKDFEEPGHL	51–62
12	FEEPGHLAPTGL	56–67	D-P
13	HLAPTGLHLGGI	61–72
14	GLHLGGIKYMVI	66–77
15	GIKYMVIQGEAG	71–82
16	VIQGEAGAVIRG	76–87	N
17	AGAVIRGKKGSG	81–92	D-P/N
18	RGKKGSGGITIK	86–97	D-P/N
19	SGGITIKKTGQA	91–102	D-P/N
20	IKKTGQALVFGI	96–107	D-P/N
21	ALVFGIYEEPVT	102–113	D-P
22	GIYEEPVTPGQC	106–117
23	TPVTPGQCNMVV	110–122
24	GQCNMVVERLGD	115–126
25	VERLGDYLIDQGL	121–133	N