Molecular Cloning, Expression, Purification, and Functional Characterization of Dammarenediol Synthase from Panax ginseng

Figure 1

SDS-PAGE analysis of the DS expression in E.coli. Lane M: molecular weight marker; Lane 1: crude cells extracts of uninduced E. coli Rosetta containing pET-30a; Lane 2: crude cell extracts after 4 h past the induction without IPTG of E. coli Rosetta containing pET-30a-His-DS; Lane 3–9: crude cell extracts after 4 h past the induction with IPTG of E. coli Rosetta containing pET-30a-His-DS. The molecular weights of the new protein components agree well with those predicted for the fusion proteins.