Microsphere Suspension Array Assays for Detection and Differentiation of Hendra and Nipah Viruses
Table 1
Oligonucleotides for HeV and NiV microsphere array assays.
Name
Function
Sequence (5′-3′)
Position (a)
N-gene
D-358
PCR-Fwd
TTTGAMGAGGCGGCTAGTTT
125–144
D-368
PCR-Rev
CATCAARCCTTCCATCTCCTC
499–479
D-676
TSPE-henipa (*MTAG-A057)
(*)-GCRGCAACWGCTACTTTGAC
188–207
D-679
TSPE-HeV (*MTAG-A061)
(*)-ACTAATAGCCCAGAACTGAGATG
236–258
D-680
TSPE-NiV (*MTAG-A067)
(*)-ACTAATAGTCCAGAGCTCAGATG
236–258
P-gene
D-550
PCR-Fwd
ACATACAACTGGACCCARTGGTT
2698–2720
D-551
PCR-Rev
CACCCTCTCTCAGGGCTTGA
2794–2775
D-641
TSPE Henipa (*MTAG-A051)
(*)-ACAGACGTTGTATACCATG
2721–2739
(a) Positions are relative to the HeV genome (GenBank accession number: NC_001906). TSPE denotes target-specific primer extension. (*) denotes sequence of 3′ extension containing anti-TAG sequence complementary to TAG sequence on microsphere as defined by particular MTAG-A# (Luminex Corporation, USA).
