Figure 5: Ammonia measurements in 2-D and 3-D control as well as α-KG containing samples were done by indophenols method. Delivery of α-KG was done in three different ways. (i) Exogenously in free form in 2-D. (ii) Chitosan or gelatin microspheres containing α-KG in both 2-D and 3-D system. (iii) α-KG containing chitosan or gelatin microspheres incorporated in the synthesized chitosan-gelatin-polypyrrole cryogel. All the experiments were performed in triplicates, and the Student’s-t test was performed to obtain the value. .