Antigenicity and Immunogenicity of Rotavirus VP6 Protein Expressed on the Surface of Lactococcus lactis

Figure 2

Optimization of VP6-CWA expression and cellular localization analysis. Western blot analysis of (a) protoplast (PP) and cell wall (CW) protein fractions of cultures of L. lactis NZ9000/pCWA:VP6 obtained two hours after induction with 100 ng/mL of nisin after reaching different OD₆₀₀. (b) Protoplast (top blots) and cell wall (bottom blots) protein fractions of cultures of L. lactis NZ9000/pCWA:VP6 induced at 0.5 OD₆₀₀ for 2 h with increasing concentrations of nisin (left blots) and induced at 0.5 OD₆₀₀ with 100 ng/mL of nisin at different time points (right blots). (c) Graph showing OD₆₀₀ at different time points after induction, to analyze the effect of different nisin concentrations (in ng/mL) on the growth of L. lactis NZ9000/pCWA:VP6.

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