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BioMed Research International
Volume 2013 (2013), Article ID 307602, 6 pages
Research Article

Preparation, Modification, and Characterization of Alginate Hydrogel with Nano-/Microfibers: A New Perspective for Tissue Engineering

1Nucleus of Cellular and Tecidual Biology (NCTBio), Post-Graduate Program in Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves 457, Centro, 96015-560 Pelotas, RS, Brazil
2Department of Operative Dentistry School of Dentistry, Federal University of Pelotas, Rua Gonçalves Chaves 457, Centro, 96015-560 Pelotas, RS, Brazil
3Department of Operative Dentistry School of Dentistry, University North of Paraná (UNOPAR), Rua Marselha, Jardim Piza, 86041-140 Londrina, PR, Brazil
4Technology Development Center, Federal University of Pelotas, Rua Felix da Cunha 809, Centro, 96010-00 Pelotas, RS, Brazil

Received 16 March 2013; Accepted 10 May 2013

Academic Editor: Kacey Gribbin Marra

Copyright © 2013 Bianca Palma Santana et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.


We aimed to develop an alginate hydrogel (AH) modified with nano-/microfibers of titanium dioxide (nfTD) and hydroxyapatite (nfHY) and evaluated its biological and chemical properties. Nano-/microfibers of nfTD and nfHY were combined with AH, and its chemical properties were evaluated by FTIR spectroscopy, X-ray diffraction, energy dispersive X-Ray analysis, and the cytocompatibility by the WST-1 assay. The results demonstrate that the association of nfTD and nfHY nano-/microfibers to AH did not modified the chemical characteristics of the scaffold and that the association was not cytotoxic. In the first 3 h of culture with NIH/3T3 cells nfHY AH scaffolds showed a slight increase in cell viability when compared to AH alone or associated with nfTD. However, an increase in cell viability was observed in 24 h when nfTD was associated with AH scaffold. In conclusion our study demonstrates that the combination of nfHY and nfTD nano-/microfibers in AH scaffold maintains the chemical characteristics of alginate and that this association is cytocompatible. Additionally the combination of nfHY with AH favored cell viability in a short term, and the addition of nfTD increased cell viability in a long term.