Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction
Figure 5
Real-time RT-PCR for TGF-β, BMP-7, Smad3, Smad8, and mRNA expression in vivo after rhEPO treatment in UUO model. TGF-β, Smad3, and mRNA expression show markedly progressive upregulation in UUO mice on days 3, 7, and 14 () than in sham or sham + rhEPO. Treatment with rhEPO significantly downregulation of TGF-β, Smad3, and mRNA expression compared with placebo treatment (). On the other hand, BMP-7 and Smad8 mRNA expression were significantly lower in UUO mice on days 3, 7, and 14 () than in sham or sham + rhEPO. Treatment with rhEPO showed significantly the sluggish downregulation of BMP-7 and Smad8 expression (). in each group. Each bar represents the mean ± SD. * versus sham group; # versus UUO group.
