Dual Inhibiting Senescence and Epithelial-to-Mesenchymal Transition by Erythropoietin Preserve Tubular Epithelial Cell Regeneration and Ameliorate Renal Fibrosis in Unilateral Ureteral Obstruction

Table 1

Sequences of real-time PCR primers and probes.

Gene	Sequence
BMP-7	Forward 5′-TGGATGGGCAGAGCATCAA-3′; Reverse 5′-CTTGGAG CGATTCTGGCTG-3′; Probe 5′-FAM-ATTGGACGGCATGGACCCCAGA-TAMRA-3′
TGF-𝛽1	Forward 5′-GGCTACCATGCCAACCAGCCTGGTGTACTCA-3′; Reverse 5′-CCGGGTTGTGTTGGTTGTAGA-3′; Probe 5′-FAM-CACACAGTACAGCAAGGTCCTTGCCCT-TAMRA-3′
Smad3	Forward 5′-GGGCCTACTGTCCAATGTCA-3′; Reverse 5′-CCCAATGTGTCGCCTTGTA-3′; Probe 5′-FAM-CCGGAATGCAGCCGTGGAAC-TAMRA-3′
Smad8	Forward 5′-CCTATCAACACTCAGACTTCCG-3′; Reverse 5′-GTGAAGCCGTCTATGAGCAC -3′; Probe 5′-FAM-ACTTTCCAGGCGTCCTCGCG-TAMRA-3′
P16^INK4a	Forward 5′-GGACACCTTGAAGGAGGAGAAAG-3′; Reverse 5′-TTCTCCAACTCCTGGATGATGA-3′; Probe 5′-FAM-CCTTCAAGGCTTGGTTTCTCGTCAGACA-TAMRA-3′
HPRT	Forward 5′-TGACACTGGTAAAACAATGCAAACT-3′; Reverse 5′-AACAAAGTCTGGCCTGTATCCAA-3′; Probe 5′-FAM-TTCACCAGCAAGCTTGCAACCTTAACC-TAMRA-3′