Stat3 Upregulates Leucine-Rich Repeat-Containing G Protein-Coupled Receptor 4 Expression in Osteosarcoma Cells

Figure 4

Stat3 activation promotes nuclear accumulation of β-catenin. (a-b) Nuclear β-catenin expression was determined in MG63 (a) and U2OS (b) cells transfected with plasmids containing empty vector (EV) or Stat3 for 36 hr. Lamin B levels were measured as a loading control. (c-d) mRNA levels of Cyclin D1 and Cyclin E were examined in MG63 (c) and U2OS (d) cells overexpressing empty vector (EV) or Stat3 for 24 hr. (e-f) Nuclear β-catenin expression was determined in MG63 (e) and U2OS (f) cells transfected with plasmids containing empty vector (EV) or Stat3 for 36 hr. Cells were pretransfected with siRNA oligos targeting LGR4 or negative controls (NC) for 16 hr. ^∗∗𝑃<0.01; ^∗∗∗𝑃<0.001.

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