Review Article
Preparative Purification of Recombinant Proteins: Current Status and Future Trends
Table 1
A panel of commonly used affinity tags selected for purification of recombinant fusion proteins and their associated characteristics.
| Tag | Size [amino acids or kDa] | Ligand or separation method | Referencea |
|
Polyhistidine | 5–15 a.a. | IMAC | [6] | HA-tag | 9 a.a. | mAb based | [7] | FLAG | 8 a.a. | mAb based | [8] | Strep tag I | 9 a.a. | Streptavidin | [9] | Strep tag II | 8 a.a. | Streptactin | [10] | Softag 1 | 13 a.a. | mAb based | [11] | Softag 3 | 8 a.a. | mAb based | [12] | T7-tag |
11–16 | mAb based | [13] | c-myc | 10 a.a. | mAb based | [14] | S-peptide | 15 a.a. | S-protein | [15] |
Polyaspartic acid | 5–16 a.a. | Ion-exchange or precipitation | [16] | VSV tag | 11 a.a. | mAb based | [17] | Calmodulin binding peptide | 26 a.a. | Calmodulin | [18] | Glutathione S-transferase | 26 kDa | Glutathione | [19] | Maltose binding domain | 40 kDa | Maltose, amylose | [20] | PinPoint (Promega) | 13 kDa | Streptavidin/avidin | [21] | Cellulose binding domain (Novagen) | 27–189 a.a. | Cellulose | [14] | Xylanase 10A | 163 a.a. | Cellulose | [22] |
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Only one relevant reference is given.
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