Oral Immunogenicity of Plant-Made Mycobacterium tuberculosis ESAT6 and CFP10
Figure 3
Electrophoretic pattern (1.5% agarose gel) of the PCR products amplified using the primers specific for the esat6 (a) gene: (1) DNA of nontransgenic carrot plant; (2–6) DNAs of the carrot plants transformed with the plasmid pBI121 carrying the esat6 gene; (7) DNA of the plasmid pBI121 carrying the esat6 gene; (8) DNA marker (SibEnzim, Novosibirsk, Russia), pBluescript II SK(+) hydrolyzed with the MspI restriction endonuclease; and (9) negative control (without DNA template); for cfp10(b): (1–7) DNAs of the carrot plants transformed with the plasmid. pBI121 carrying the cfp10 gene; (8) DNA of nontransgenic carrot plant; (9) DNA of the plasmid. pBI121 carrying the cfp10 gene; (10) negative control (without DNA template); and (11) DNA marker (SibEnzim, Novosibirsk, Russia).
