Association of Toll-Like Receptor 4 Polymorphisms with Diabetic Foot Ulcers and Application of Artificial Neural Network in DFU Risk Assessment in Type 2 Diabetes Patients
Figure 1
Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analysis of five SNPs of the Toll-like receptor 4 (TLR4) gene. (a) For genotyping rs4986790, the 140 bp PCR product was digested with BccI. The A allele is not cut by the enzyme, whereas the G allele yields 77 and 63 bp products. (b) For genotyping rs4986791, the 110 bp PCR product was digested with BslI. The T allele is not cut by the enzyme, whereas the C allele yields 89 and 21 bp products. (c) For genotyping rs1927911, the 203 bp PCR product was digested with StyI. The T allele is not cut by the enzyme, whereas the C allele yields 178 and 25 bp products. (d) For genotyping rs11536858, the 241 bp PCR product was digested with KpnI. The A allele is not cut by the enzyme, whereas the G allele yields 190 and 51 bp products. (e) For genotyping rs1927914, the 157 bp PCR product was digested with SphI. The T allele is not cut by the enzyme, whereas the C allele yields 90 and 67 bp products.
