BioMed Research International / 2013 / Article / Fig 3

Research Article

DHA Inhibits Protein Degradation More Efficiently than EPA by Regulating the PPARγ/NFκB Pathway in C2C12 Myotubes

Figure 3

Effects of n-3 PUFA on PPARγ gene expression. C2C12 myotubes were incubated with 600 μM EPA, 600 μM DHA, or 600 μM BSA for 24 hours. The PPARγ mRNA was determined using real-time PCR analysis and relative abundance of mRNA was calculated after normalization to β-actin. PPARγ = peroxisome proliferator-activated receptor γ, BSA  =  bovine serum albumin, DHA  =  docosahexaenoic acid, and EPA= eicosapentaenoic acid. Data are expressed as mean ± S.D. of 3 different experiments. ** versus control.

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