Regulatory Interactions between Androgens, Hoxb5, and TGFβ Signaling in Murine Lung Development
Figure 5
In vivo DHT treatment altered Hoxb5 cellular expression and protein levels. (a) Control E18 lungs exhibited a gradient of Hoxb5 localization (blue nuclear staining) with minimal mesenchymal expression in central lung (#) and increased expression in peripheral regions, especially around developing saccules (*). (b) In DHT-treated lungs the Hoxb5 mesenchymal expression pattern lacked the normal central-to-peripheral gradient. Terminal sacs were less developed appearing rounder with few developing septa seen, indicating immaturity of airway patterning. 40x Mag in (a), (b). (c) Hoxb5 protein levels increased 3-fold with DHT treatment compared to control lungs. *, mean ± SEM, .
